APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Target Retrieval Solution buffer.
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Not tested in other applications.
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Cat, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 2% BSA
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.22 mg/ml
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase 3,CPP32,CPP32B,SCA-1
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX639931 IHC-P Image
IHC-P analysis of human cervical adenocarcinoma tissue using GTX639931 Caspase 3 antibody [HMV307] HistoMAX™. Adenocarcinoma with strong caspase 3 staining of tumor cells.
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GTX639931 IHC-P Image
IHC-P analysis of human gastric antrum tissue using GTX639931 Caspase 3 antibody [HMV307] HistoMAX™. Moderate to strong cytoplasmic caspase 3 positivity of epithelial cells of the surface epithelium.
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GTX639931 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX639931 Caspase 3 antibody [HMV307] HistoMAX™. Variable cytoplasmic caspase 3 positivity of lymphocytic cells and staining is strongest in germinal centres.
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REFERENCE
There are currently no references for Caspase 3 antibody [HMV307] HistoMAX™ (GTX639931). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Caspase 3 antibody [HMV307] HistoMAX™ (GTX639931). Be the first to share your experience with this product.